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  • Journal article
    Cho KH, Byrne B, Chae PS, 2013,

    , CHEMBIOCHEM, Vol: 14, Pages: 452-455, ISSN: 1439-4227
    • Cite
    • Citations: 31
  • Journal article
    Curry S, 2013,

    , Insights, Vol: 26, Pages: 22-27, ISSN: 2048-7754
  • Journal article
    Chappell J, Jensen K, Freemont PS, 2013,

    , Nucleic Acids Research, Vol: 41, Pages: 3471-3481, ISSN: 0305-1048

    A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which are inherently time-consuming. To address this, we have validated a completely in vitro approach for the characterization of DNA regulatory elements using Escherichia coli extract cell-free systems. Importantly, we demonstrate that characterization in cell-free systems correlates and is reflective of performance in vivo for the most frequently used DNA regulatory elements. Moreover, we devise a rapid and completely in vitro method to generate DNA templates for cell-free systems, bypassing the need for DNA template generation and amplification from living cells. This in vitro approach is significantly quicker than current characterization methods and is amenable to high-throughput techniques, providing a valuable tool for rapidly prototyping libraries of DNA regulatory elements for synthetic biology.
  • Journal article
    Bill R, Byrne B, 2013,

    , MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 113-113, ISSN: 0968-7688
  • Journal article
    Faunce TA, Lubitz W, Rutherford AWB, MacFarlane DR, Moore GF, Yang P, Nocera DG, Moore TA, Gregory DH, Fukuzumi S, Yoon KB, Armstrong FA, Wasielewski MR, Styring Set al., 2013,

    , ENERGY & ENVIRONMENTAL SCIENCE, Vol: 6, Pages: 695-698, ISSN: 1754-5692
    • Cite
    • Citations: 250
  • Journal article
    Ruggiero A, Marchant J, Squeglia F, Makarov V, De Simone A, Berisio Ret al., 2013,

    , JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, Vol: 31, Pages: 195-205, ISSN: 0739-1102
    • Cite
    • Citations: 30
  • Journal article
    Xu Y, Matthews S, 2013,

    , JOURNAL OF BIOMOLECULAR NMR, Vol: 55, Pages: 179-187, ISSN: 0925-2738
    • Cite
    • Citations: 37
  • Journal article
    Wang GD, Heng JYY, Williams DR, 2013,

    , POWDER TECHNOLOGY, Vol: 236, Pages: 12-16, ISSN: 0032-5910
  • Journal article
    Delmas T, Shah UV, Roberts MM, Williams DR, Heng JYYet al., 2013,

    , POWDER TECHNOLOGY, Vol: 236, Pages: 24-29, ISSN: 0032-5910
    • Cite
    • Citations: 17
  • Journal article
    Khoo JY, Shah UV, Schaepertoens M, Williams DR, Heng JYYet al., 2013,

    , POWDER TECHNOLOGY, Vol: 236, Pages: 114-121, ISSN: 0032-5910
    • Cite
    • Citations: 23
  • Journal article
    Bill R, Byrne B, 2013,

    , MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 2-2, ISSN: 0968-7688
  • Journal article
    Porta C, Xu X, Loureiro S, Paramasivam S, Ren J, Al-Khalil T, Burman A, Jackson T, Belsham GJ, Curry S, Lomonossoff GP, Parida S, Paton D, Li Y, Wilsden G, Ferris N, Owens R, Kotecha A, Fry E, Stuart DI, Charleston B, Jones IMet al., 2013,

    , JOURNAL OF VIROLOGICAL METHODS, Vol: 187, Pages: 406-412, ISSN: 0166-0934
    • Cite
    • Citations: 52
  • Journal article
    Solomon A, Smyth E, Mitha N, Pitchford S, Vydyanath A, Luther PK, Thorley AJ, Tetley TD, Emerson Met al., 2013,

    , JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 11, Pages: 325-334, ISSN: 1538-7933
    • Cite
    • Citations: 23
  • Conference paper
    Zhao Z, Rutherford AW, Moser CC, Dutton PLet al., 2013,

    , 57th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 489A-489A, ISSN: 0006-3495
  • Journal article
    Simon AC, Simpson PJ, Goldstone RM, Krysztofinska EM, Murray JW, High S, Isaacson RLet al., 2013,

    , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 1327-1332, ISSN: 0027-8424
    • Cite
    • Citations: 28
  • Journal article
    Saito K, Rutherford AW, Ishikita H, 2013,

    , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 954-959, ISSN: 0027-8424
    • Cite
    • Citations: 133
  • Journal article
    Mesquita FS, Holden DW, Rolhion N, 2013,

    , PLOS ONE, Vol: 8, ISSN: 1932-6203
    • Cite
    • Citations: 19
  • Journal article
    Thapper A, Styring S, Saracco G, Rutherford AW, Robert B, Magnuson A, Lubitz W, Llobet A, Kurz P, Holzwarth A, Fiechter S, De Groot H, Campagna S, Braun A, Bercegol H, Artero Vet al., 2013,

    , Green, Vol: 3, Pages: 43-57, ISSN: 1869-876X

    On the path to an energy transition away from fossil fuels to sustainable sources, the European Union is for the moment keeping pace with the objectives of the Strategic Energy Technology-Plan. For this trend to continue after 2020, scientific breakthroughs must be achieved. One main objective is to produce solar fuels from solar energy and water in direct processes to accomplish the efficient storage of solar energy in a chemical form. This is a grand scientific challenge. One important approach to achieve this goal is Artificial Photosynthesis. The European Energy Research Alliance has launched the Joint Programme "Advanced Materials & Processes for Energy Applications" (AMPEA) to foster the role of basic science in Future Emerging Technologies. European researchers in artificial photosynthesis recently met at an AMPEA organized workshop to define common research strategies and milestones for the future. Through this work artificial photosynthesis became the first energy research sub-field to be organised into what is designated "an Application" within AMPEA. The ambition is to drive and accelerate solar fuels research into a powerful European field - in a shorter time and with a broader scope than possible for individual or national initiatives. Within AMPEA the Application Artificial Photosynthesis is inclusive and intended to bring together all European scientists in relevant fields. The goal is to set up a thorough and systematic programme of directed research, which by 2020 will have advanced to a point where commercially viable artificial photosynthetic devices will be under development in partnership with industry.
  • Journal article
    Bebeacua C, Lai L, Vegge CS, Brondsted L, van Heel M, Veesler D, Cambillau Cet al., 2013,

    , JOURNAL OF VIROLOGY, Vol: 87, Pages: 1061-1068, ISSN: 0022-538X
    • Cite
    • Citations: 56
  • Journal article
    Metifiot M, Maddali K, Johnson BC, Hare S, Smith SJ, Zhao XZ, Marchand C, Burke TR, Hughes SH, Cherepanov P, Pommier Yet al., 2013,

    , ACS CHEMICAL BIOLOGY, Vol: 8, Pages: 209-217, ISSN: 1554-8929
    • Cite
    • Citations: 42
  • Journal article
    Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SMet al., 2013,

    , NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 20, Pages: 99-U126, ISSN: 1545-9993
    • Cite
    • Citations: 180
  • Book
    Matthews S, Heise H, 2013,

    Modern NMR Methodology Preface

    , Publisher: SPRINGER-VERLAG BERLIN
  • Journal article
    Chappell J, Freemont P, 2013,

    , SYNTHETI C BIOLOGY, Vol: 1073, Pages: 61-74, ISSN: 1064-3745
  • Journal article
    Juozapavicius M, Kaucikas M, Dimitrov SD, Barnes PRF, van Thor JJ, O'Regan BCet al., 2013,

    Evidence for “Slow” Electron Injection in Commercially Relevant Dye-2 Sensitized Solar Cells by Vis−NIR and IR Pump−Probe Spectroscopy

    , J Phys Chem C

    We present femtosecond to nanosecond transient absorption (TA)data on electron injection in dye-sensitized solar cells (DSSCs) fabricated with lowvolatility, commercially relevant electrolytes, with and without added lithium.Results are shown over an extended time range (300 fs−6.3 ns) and extendedwavelength range (800−1400 nm) for both N719 and C106 dyes. Kinetics weremeasured on both TiO2 and noninjecting ZrO2. Using the latter, we havedetermined the spectra and absorption coefficient of N719* across the wavelengthrange. We find an isosbestic point in the TA spectra on TiO2 near 900 nm for allcells, existing from <1 ps to >1 ns. We show how measurements near this isosbesticpoint can give a false impression of uniformly femtosecond injection dynamics inDSSCs. Comparison of dynamics measured at 1200 nm with mid-IR transient absorption measured at 5100 nm confirms amajority proportion of slow (>10 ps) electron injection in these commercially relevant cells. We also comment on a recentpublication which appears to directly contradict the results we present.
  • Book chapter
    Goers L, Kylilis N, Tomazou M, Wen KY, Freemont P, Polizzi Ket al., 2013,

    , MICROBIAL SYNTHETIC BIOLOGY, Editors: Harwood, Wipat, Publisher: ELSEVIER ACADEMIC PRESS INC, Pages: 119-156, ISBN: 978-0-12-417029-2
    • Cite
    • Citations: 13
  • Journal article
    Kaucikas M, Warren M, Michailovas A, Antanavicius R, van Thor JJet al., 2013,

    , Laser Physics, Pages: 1-6
  • Book chapter
    Choudhury HG, Beis K, 2013,

    Tellurite-Resistance Protein TehA from Escherichia coli

    , Encyclopedia of Metalloproteins, Editors: Kretsinger, Uversky, Permyakov, Publisher: Springer, ISBN: 978-1-4614-1532-9
  • Journal article
    Kaucikas M, Barber J, van Thor JJ, 2013,

    , Optics Express, Vol: 21, Pages: 8357-8370
  • Book chapter
    Kim TW, Lee JH, Choi J, Kim KH, van Wilderen LGW, Guerin L, Kim Y, Yung YO, Yang C, Kim J, Wulff M, van Thor JJ, Ihee Het al., 2013,

    Unravelling the protein structural dynamics of photoactive yellow protein in solution using pump-probe X-ray solution scattering
  • Journal article
    Boehm CR, Freemont PS, Ces O, 2013,

    , LAB ON A CHIP, Vol: 13, Pages: 3426-3432, ISSN: 1473-0197
    • Cite
    • Citations: 26

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