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• Journal article
  McCarthy NLC, Ces O, Law RV, Seddon JM, Brooks NJet al., 2015,
  , Chem Commun (Camb), Vol: 51, Pages: 8675-8678

  We have imaged the formation of membrane microdomains immediately after their induction using a novel technology platform coupling high hydrostatic pressure to fluorescence microscopy. After formation, the ordered domains are small and highly dynamic. This will enhance links between model lipid assemblies and dynamic processes in cellular membranes.

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• Journal article
  Elani Y, Law RV, Ces O, 2015,
  , THERAPEUTIC DELIVERY, Vol: 6, Pages: 541-543, ISSN: 2041-5990
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  • Citations: 13
• Journal article
  Branch T, Girvan P, Barahona M, Ying Let al., 2015,
  , ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 54, Pages: 1227-1230, ISSN: 1433-7851
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  • Citations: 46
• Journal article
  Barriga HMG, Booth P, Haylock S, Bazin R, Templer RH, Ces Oet al., 2014,
  , Journal of the Royal Society Interface, Vol: 11, ISSN: 1742-5662

  Droplet interface bilayers (DIBs) provide an exciting new platform for the study of membrane proteins in stable bilayers of controlled composition. To date, the successful reconstitution and activity measurement of membrane proteins in DIBs has relied on the use of the synthetic lipid 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC). We report the functional reconstitution of the mechanosensitive channel of large conductance (MscL) into DIBs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), a lipid of significantly greater biological relevance than DPhPC. MscL functionality has been demonstrated using a fluorescence-based assay, showing that dye flow occurs across the DIB when MscL is gated by the cysteine reactive chemical 2-(trimethylammonium)ethyl methane thiosulfonate bromide (MTSET). MscL has already been the subject of a number of studies investigating its interaction with the membrane. We propose that this method will pave the way for future MscL studies looking in detail at the effects of controlled composition or membrane asymmetry on MscL activity using biologically relevant lipids and will also be applicable to other lipid–protein systems, paving the way for the study of membrane proteins in DIBs with biologically relevant lipids.

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• Conference paper
  Nickdel MB, Lagarto JL, Kelly DJ, Manning HB, Yamamoto K, Talbot CB, Dunsby C, French P, Itoh Yet al., 2014,
  , Conference on Optical Biopsy XII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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• Journal article
  Mak LH, Knott J, Scott KA, Scott C, Whyte GF, Ye Y, Mann DJ, Ces O, Stivers J, Woscholski Ret al., 2012,
  , BIOORGANIC & MEDICINAL CHEMISTRY, Vol: 20, Pages: 4371-4376, ISSN: 0968-0896
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  • Citations: 13
• Journal article
  Wormit A, Butt SM, Chairam I, McKenna JF, Nunes-Nesi A, Kjaer L, O'Donnelly K, Fernie AR, Woscholski R, Barter MCL, Hamann Tet al., 2012,
  , PLANT PHYSIOLOGY, Vol: 159, Pages: 105-117, ISSN: 0032-0889
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  • Citations: 57
• Journal article
  Charalambous K, Booth PJ, Woscholski R, Seddon JM, Templer RH, Law RV, Barter LMC, Ces Oet al., 2012,
  , JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 134, Pages: 5746-5749, ISSN: 0002-7863
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  • Citations: 34
• Journal article
  Furse S, Brooks NJ, Seddon AM, Woscholski R, Templer RH, Tate EW, Gaffney PRJ, Ces Oet al., 2012,
  , Soft Matter
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• Journal article
  Mak LH, Georgiades SN, Rosivatz E, Whyte GF, Mirabelli M, Vilar R, Woscholski Ret al., 2011,
  , ACS Chemical Biology, ISSN: 1554-8929
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