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BibTex format

@article{Robinson:2020:10.1016/j.ymben.2020.04.008,
author = {Robinson, CJ and Carbonell, P and Jervis, AJ and Yan, C and Hollywood, KA and Dunstan, MS and Currin, A and Swainston, N and Spiess, R and Taylor, S and Mulherin, P and Parker, S and Rowe, W and Matthews, NE and Malone, KJ and Le, Feuvre R and Shapira, P and Barran, P and Turner, NJ and Micklefield, J and Breitling, R and Takano, E and Scrutton, NS},
doi = {10.1016/j.ymben.2020.04.008},
journal = {METABOLIC ENGINEERING},
pages = {168--182},
title = {Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers},
url = {http://dx.doi.org/10.1016/j.ymben.2020.04.008},
volume = {60},
year = {2020}
}
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TY  - JOUR
AB  - Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities of a biomanufacturing pipeline to enable rapid prototyping of microbial cell factories for the production of chemically diverse industrially relevant material building blocks. Over 85 days the pipeline was able to produce 17 potential material monomers and key intermediates by combining 160 genetic parts into 115 unique biosynthetic pathways. To explore the scale-up potential of our prototype production strains, we optimized the enantioselective production of mandelic acid and hydroxymandelic acid, achieving gram-scale production in fed-batch fermenters. The high success rate in the rapid design and prototyping of microbially-produced material building blocks reveals the potential role of biofoundries in leading the transition to sustainable materials production.
AU  - Robinson,CJ
AU  - Carbonell,P
AU  - Jervis,AJ
AU  - Yan,C
AU  - Hollywood,KA
AU  - Dunstan,MS
AU  - Currin,A
AU  - Swainston,N
AU  - Spiess,R
AU  - Taylor,S
AU  - Mulherin,P
AU  - Parker,S
AU  - Rowe,W
AU  - Matthews,NE
AU  - Malone,KJ
AU  - Le,Feuvre R
AU  - Shapira,P
AU  - Barran,P
AU  - Turner,NJ
AU  - Micklefield,J
AU  - Breitling,R
AU  - Takano,E
AU  - Scrutton,NS
DO  - 10.1016/j.ymben.2020.04.008
EP  - 182
PY  - 2020///
SN  - 1096-7176
SP  - 168
TI  - Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers
T2  - METABOLIC ENGINEERING
UR  - http://dx.doi.org/10.1016/j.ymben.2020.04.008
VL  - 60
ER  -
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