In this section
@article{Bozhuyuk:2019:10.1016/j.mib.2019.10.007,
author = {Bozhuyuk, KAJ and Micklefield, J and Wilkinson, B},
doi = {10.1016/j.mib.2019.10.007},
journal = {CURRENT OPINION IN MICROBIOLOGY},
pages = {88--96},
title = {Engineering enzymatic assembly lines to produce new antibiotics},
url = {http://dx.doi.org/10.1016/j.mib.2019.10.007},
volume = {51},
year = {2019}
}
TY - JOUR
AB - Numerous important therapeutic agents, including widely-used antibiotics, anti-cancer drugs, immunosuppressants, agrochemicals and other valuable compounds, are produced by microorganisms. Many of these are biosynthesised by modular enzymatic assembly line polyketide synthases, non ribosomal peptide synthetases, and hybrids thereof. To alter the backbone structure of these valuable but difficult to modify compounds, the respective enzymatic machineries can be engineered to create even more valuable molecules with improved properties and/or to bypass resistance mechanisms. In the past, many attempts to achieve assembly line pathway engineering failed or led to enzymes with compromised activity. Recently our understanding of assembly line structural biology, including an appreciation of the conformational changes that occur during the catalytic cycle, have improved hugely. This has proven to be a driving force for new approaches and several recent examples have demonstrated the production of new-to-nature molecules, including anti-infectives. We discuss the developments of the last few years and highlight selected, illuminating examples of assembly line engineering.
AU - Bozhuyuk,KAJ
AU - Micklefield,J
AU - Wilkinson,B
DO - 10.1016/j.mib.2019.10.007
EP - 96
PY - 2019///
SN - 1369-5274
SP - 88
TI - Engineering enzymatic assembly lines to produce new antibiotics
T2 - CURRENT OPINION IN MICROBIOLOGY
UR - http://dx.doi.org/10.1016/j.mib.2019.10.007
VL - 51
ER -