51�Թ���

Lead: Professor Yuri Korchev, Dr Andriy Shevchuk

We advance next-generation SICM platforms for non-contact nanoscale imaging of living cells. Our work focuses on probe engineering, high-speed feedback control, multimodal integration and quantitative analysis of membrane dynamics.

Lead: Professor Yuri Korchev, Dr Andriy Shevchuk

We develop multifunctional nanopipettes for electrochemical sensing, molecular delivery and minimally invasive single-cell sampling. These tools enable localised measurement of ions, metabolites and signalling molecules directly at the cell surface.

Lead: Dr Andriy Shevchuk, Professor Yuri Korchev

We integrate SICM with advanced fluorescence microscopy to correlate nanoscale topography with molecular dynamics. This multimodal approach enables quantitative mapping of live-cell processes in real time.

Lead: Dr Andriy Shevchuk, Professor Yuri Korchev

Our laboratory develops electrochemical approaches to probe redox biology, membrane transport and cellular signalling at single-cell resolution.

Lead: Professor Yuri Korchev, Dr Andriy Shevchuk

We apply physical modelling and computational analysis to understand membrane mechanics, microdomain organisation and disease-relevant cellular behaviour.

The Nanomedicine Laboratory aims to establish nanoscale functional mapping of living cells as a foundational technology for precision medicine. By unifying physics-based instrumentation, electrochemistry, and advanced microscopy, we seek to transform how cellular dysfunction is detected, quantified and therapeutically targeted. Our long-term vision is to develop minimally invasive single-cell platforms that enable real-time diagnostic and mechanistic insight across cancer, cardiometabolic disease and inflammatory disorders.

WPI-NanoLSI, Japan

The Nanomedicine Laboratory contributes to the mission of interdisciplinary nano-life science by developing enabling technologies that connect nanoscale physical measurements with biological function. Our work integrates physics, engineering and biomedical research to visualise and quantify living systems at nanometre resolution.

Through collaborations spanning 51�Թ��� and international nano-science centres, we advance multimodal nanopipette platforms capable of structural imaging, electrochemical sensing and molecular sampling. This cross-disciplinary approach supports mechanistic understanding of complex cellular systems and promotes the translation of nanotechnology into life-science applications.

(Representative recent and landmark contributions;

1. Fabio Marcuccio, Philip S Goff, Devkee M Vadukul, Fawaz Raja, Yilin Li, Ren Ren, Debjani Saha, Luca Magnani, Francesco A Aprile, Uma Anand, Elena V Sviderskaya, Joshua B Edel, Aleksandar P Ivanov, Petr V Gorelkin, Yuri Korchev, Andrew Shevchuk. ()2025 Delivery of Biomolecules into Individual Cells and Subcellular Compartments by Localized Electroporation via Nanopipette. ACS Nanoscience Au, 5 (5), 353-361
2. D Wang, E Woodcock, X Yang, H Nishikawa, EV Sviderskaya, M Oshima, C Edwards, Y Zhang and Y Korchev (2024) Exploration of individual colorectal cancer cell responses to H2O2 eustress using hopping probe scanning ion conductance microscopy. Science Bulletin,
3. S Cai, R Ren, J He, X Wang, Z Zhang, Z Luo, W Tan, Y Korchev, JB Edel, AP Ivanov. (2023) Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids. Nano Letters 23 (24), 11438-11446
4. R Ren, S Cai, X Fang, X Wang, Z Zhang, M Damiani, C Hudlerova, A Rosa, J Hope, N Cook, P Gorelkin, A Erofeev, P Novak, A Badhan, M Crone, P Freemont, GP Taylor, L Tang, C Edwards, A Shevchuk, P Cherepanov, Z Luo, W Tan, Y Korchev, AP Ivanov, JB Edel. (2023) Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes. Nature Comm 14(1) 7362
5. F Liu, M Gledhill, Q-G Tan, K Zhu, Q Zhang, P Salaün, A Tagliabue, Y Zhang, D Weiss, EP Achterberg, Y Korchev, (2022) Phycosphere pH of unicellular nano-and micro-phytoplankton cells and consequences for iron speciation. The ISME journal, 1-8
6. Bednarska, J; Pelchen-Matthews, A; Novak, P; Burden, JJ; Summers, PA; Kuimova, MK; Korchev, Y; Marsh, M; Shevchuk, A. (2020) Rapid formation of human immunodeficiency virus-like particles. Proc. Natl. Acad. Sci. U.S.A,
7. Zhang, Y., Takahashi, Y., Hong, S.P., Liu, F., Bednarska, J., Goff, P.S., Novak, P., Shevchuk, A., Gopal, S., Barozzi, I., L. Magnani, H. Sakai, Y. Suguru, T. Fujii, A. Erofeev, P. Gorelkin, A. Majouga, D.J. Weiss, C. Edwards, A.P Ivanov, D Klenerman, EV Sviderskaya, JB Edel and Y Korchev  (2019). High-resolution label-free 3D mapping of extracellular pH of single living cells. Nature Comm 10, 1-9.
8. Ren, R., Y. Zhang, B. P. Nadappuram, B. Akpinar, D. Klenerman, A. P. Ivanov, J. B. Edel, and Y. Korchev. (2017). Nanopore extended field-effect transistor for selective single-molecule biosensing. Nature comm. 8: 586 .
9. Y Zhang, J Clausmeyer, B Babakinejad, AL Córdoba, T Ali, A Shevchuk, Y Takahashi, P Novak, C Edwards, M Lab, S Gopal, C Chiappini, U Anand, L Magnani, RC Coombes, J Gorelik, T Matsue, W Schuhmann, D Klenerman, EV Sviderskaya, Y Korchev  (2016). Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis. ACS nano 10, 3214-3221
10. A Leo-Macias, E Agullo-Pascual, JL Sanchez-Alonso, S Keegan, X Lin, T Arcos, YE Korchev, J Gorelik, D Fenyö, E Rothenberg, M Delmar (2016). Nanoscale visualization of functional adhesion/excitability nodes at the intercalated disc. Nature comm. 7:10342. doi: 10.1038/ncomms10342
11. Y Takahashi, AI Shevchuk, P Novak, Y Zhang, N Ebejer, JV Macpherson, PR Unwin, AJ Pollard, D Roy, CA Clifford, H Shiku, T Matsue, D Klenerman, YE Korchev (2014) Corrigendum: Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces. Angew. Chem. Int. Ed Engl. 126, 7235-7235
12. Y. Takahashi, A. Kumatani, H. Munakata, H. Inomata, K. Ito, K. Ino, H. Shiku, P.R. Unwin, Y.E. Korchev, K. Kanamura, T. Matsue (2014) Nanoscale visualisation of redox activity at Li ion battery cathodes. Nature Com. doi:10.1038/ncomms6450
13. Novak, P., A. Shevchuk, P. Ruenraroengsak, M. Miragoli, A. J. Thorley, D. Klenerman, M. J. Lab, T. D. Tetley, J. Gorelik, and Y. E. Korchev. (2014). Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy. Nano. Lett. 14: 1202-1207
14. Actis, P., Tokar, S., Clausmeyer, J., Babakinejad, B., Mikhaleva, S., Cornut, R., Takahashi, Y., Cordoba, A. L., Novak, P., Shevchuck, A. I., Dougan, J. A., Kazarian, S. G., Gorelkin, P. V., Erofeev, A. S., Yaminsky, I. V., Unwin, P. R., Schuhmann, W., Klenerman, D., Rusakov, D. A., Sviderskaya, E. V., and Korchev, Y. E.( 2013) Electrochemical Nanoprobes for Single-Cell Analysis. Acs Nano 8(1), 875-884.
15. P Novak, J Gorelik, U Vivekananda, A. Shevchuk, Y. Ermolyuk, R. Bailey, A. Bushby, G. Moss, D. Rusakov, D Klenerman, D. Kullmann, K. Volynski, Y. Korchev. (2013) Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels Neuron, 79 (6), 1067-1077
16. A. Shevchuk, P. Novak, M. Taylor, I. Diakonov, A. Ziyadeh-Isleem, M. Bitoun, P. Guicheney, M. Lab, J. Gorelik, C. Merrifield, D. Klenerman and Y. Korchev. (2012) An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy. J. Cell Biol. 197 (4), 499-508
17. Y Takahashi, A Shevchuk, P Novak, B Babakinejad, J Macpherson, P Unwin, H Shiku, J Gorelik, D Klenerman, and Y Korchev*, T Matsue* (2012) Topographical and Electrochemical Nanoscale Imaging of Living Cells using Voltage Switching Mode Scanning Electrochemical Microscopy. Proc. Natl. Acad. Sci. U.S.A, 109 (29), 11540-11545
18. Y Takahashi, A Shevchuk, P Novak, Y Zhang, N Ebejer, J Macpherson, P Unwin, A Pollard, D Roy, C Clifford, H Shiku, T Matsue, D Klenerman, and Y Korchev .(2011) Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces. Angew. Chem. Int. Ed Engl. 50:9638-9642
19. V Nikolaev, A Moshkov, A Lyon, M Miragoli, P Novak, H Paur, M Lohse, Y Korchev, S Harding, J Gorelik (2010) Redistribution of ß2 adrenergic receptors leads to changes in camp compartmentation in heart failure. Science 327: 1653-1657.
20. J. Adler, A. I. Shevchuk, P. Novak, Y. E. Korchev, I. Parmryd. (2010) High Resolution Plasma Membrane Topography Imaging for Correct Interpretation of Single Particle Tracks. Nature Meth. 7: 170-171.
21. Pavel Novak, Chao Li, Andrew I. Shevchuk, Ruben Stepanyan, Matthew Caldwell, Simon Hughes, Trevor G. Smart, Julia Gorelik, Victor P. Ostanin, Max J. Lab, Guy W. J. Moss, Gregory I. Frolenkov, David Klenerman and Yuri E. Korchev. (2009) Nanoscale live cell imaging using hopping probe ion conductance microscopy. Nature Meth. 6: 279-281
22. Klenerman, D. and Y. Korchev. 2006. Potential biomedical applications of the scanned nanopipette. Nanomed. 1:107-114.
23. Shevchuk, A. I., G. I. Frolenkov, D. Sanchez, P. S. James, N. Freedman, M. J. Lab, R. Jones, D. Klenerman, and Y. E. Korchev. 2006. Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy. Angew. Chem. Int. Ed Engl. 45:2212-2216.
24. Gorelik, J., Y. Zhang, D. Sanchez, A. Shevchuk, G. Frolenkov, M. Lab, D. Klenerman, C. Edwards, and Y. Korchev. 2005. Aldosterone acts via an ATP autocrine/paracrine system: the Edelman ATP hypothesis revisited. Proc. Natl. Acad. Sci. U. S. A 102:15000-15005.
25. Gorelik, J., A. I. Shevchuk, G. I. Frolenkov, I. A. Diakonov, M. J. Lab, C. J. Kros, G. P. Richardson, I. Vodyanoy, C. R. Edwards, D. Klenerman, and Y. E. Korchev. 2003. Dynamic assembly of surface structures in living cells. Proc. Natl. Acad. Sci. U. S. A 100:5819-5822.
26. Gorelik, J., A. Shevchuk, M. Ramalho, M. Elliott, C. Lei, C. F. Higgins, M. J. Lab, D. Klenerman, N. Krauzewicz, and Y. Korchev. 2002. Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging: application to single virus-like particle entry into a cell. Proc. Natl. Acad. Sci. U. S. A 99:16018-16023.
27. Korchev, Y. E., Y. A. Negulyaev, C. R. Edwards, I. Vodyanoy, and M. J. Lab. 2000. Functional localization of single active ion channels on the surface of a living cell. Nat. Cell Biol. 2:616-619.
28. Korchev, Y. E., C. L. Bashford, M. Milovanovic, I. Vodyanoy, and M. J. Lab. 1997. Scanning ion conductance microscopy of living cells. Biophys. J. 73:653-658.