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Researchers have developed a protocol outlining the methodology for integrated DLIT-μCT imaging of a bacterial infection using the IVIS Spectrum CT

Researchers at the MRC Centre for Molecular Bacteriology and Infection and PerkinElmer have developed a protocol outlining the methodology for integrated DLIT-μCT imaging of a bacterial infection using a single multimodality imaging platform, the IVIS Spectrum CT, and the generation of a 4D movie showing the true dynamics of the C. rodentium infection cycle in mice. Published in the Journal of Visualized Experiments, this is the first documented procedure using the IVIS Spectrum CT, a new facility at the College that researchers are using to study in vivo the processes of bacterial infection.

The use of the IVIS Spectrum CT facilitates seamless co-registration of optical and μCT scans using a single imaging platform. The low dose μCT modality enables the imaging of mice at multiple time points during infection, providing detailed anatomical localization of bioluminescent bacterial foci in 3D without causing artifacts from the cumulative radiation. Importantly, the 4D movies of infected mice provide a powerful analytical tool to non-invasively monitor bacterial colonisation dynamics in vivo.

Gad Frankel, Professor of Molecular Pathogenesis with the MRC Centre for Molecular Bacteriology and Infection, and Director of the College’s imaging facility said “This technology will enable researchers to study mechanisms for bacterial infection in real time and to investigate the effectiveness of intervention strategies”.

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