BibTex format
@article{DeBerg:2026:10.1016/j.jaci.2026.02.026,
author = {DeBerg, HA and Baloh, CH and DeGottardi, Q and Hou, J and Johansson, A and Newell, E and Laidlaw, TM and Sanda, S and Shamji, M and Durham, S and Togias, A and Kwok, WW},
doi = {10.1016/j.jaci.2026.02.026},
journal = {J Allergy Clin Immunol},
pages = {845--856},
title = {Differential effects of subcutaneous and sublingual immunotherapy on timothy grass-specific TH2 CD4+ T-cell subsets.},
url = {http://dx.doi.org/10.1016/j.jaci.2026.02.026},
volume = {158},
year = {2026}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BACKGROUND: Allergen-specific CD4+ T cells are a highly heterogenous population. Depletion of these cells has been proposed as essential to achieve allergen desensitization in allergen immunotherapy. OBJECTIVE: The overall aim of this study was to characterize the heterogeneity of timothy grass (Phleum pratense) allergen-specific CD4+ T cells and determine how the frequency and phenotype of these cells change in response to sublingual (SLIT) and subcutaneous (SCIT) immunotherapy. Correlations between frequencies of these cells with Total Nasal Symptom Score and grass-specific serum immunoglobulin were also investigated. METHODS: Mass cytometry with lanthanides-tagged peptide major histocompatibility complex class II multimers and CD154 upregulation assays were used to examine changes in the frequency and phenotype of Phl p-specific CD4+ T cells in longitudinal peripheral blood mononuclear cell samples from a randomized, double-blind, placebo-controlled trial of SLIT and SCIT. Supervised and unsupervised clustering was used for data analysis. RESULTS: Phenotypes of Phl p-specific T cells were highly heterogenous but could be categorized into two major metaclusters, CRTH2hiCD27lo and CRTH2loCD27hi, each with distinct phenotypic profiles. Weak positive correlations between Total Nasal Symptom Score and frequencies of T cells within both subsets were observed. SCIT preferentially depleted CRTH2hiCD27lo cells, whereas SLIT depleted CRTH2loCD27hi cells. CRTH2hiCD27lo cell frequency correlated with Phl p-specific IgE and IgG4, but not IgA, levels. CONCLUSION: Unsupervised clustering revealed distinct subpopulations of allergen-specific T cells that were differentially targeted and depleted by SCIT and SLIT, suggesting that SCIT and SLIT act through overlapping but distinct immunologic pathways.
AU - DeBerg,HA
AU - Baloh,CH
AU - DeGottardi,Q
AU - Hou,J
AU - Johansson,A
AU - Newell,E
AU - Laidlaw,TM
AU - Sanda,S
AU - Shamji,M
AU - Durham,S
AU - Togias,A
AU - Kwok,WW
DO - 10.1016/j.jaci.2026.02.026
EP - 856
PY - 2026///
SP - 845
TI - Differential effects of subcutaneous and sublingual immunotherapy on timothy grass-specific TH2 CD4+ T-cell subsets.
T2 - J Allergy Clin Immunol
UR - http://dx.doi.org/10.1016/j.jaci.2026.02.026
UR - https://www.ncbi.nlm.nih.gov/pubmed/41780572
VL - 158
ER -