In this section
@article{Akhundova:2026,
author = {Akhundova, N and Paul, R and Fletcher, B and Connolly, M and Griffiths, M and Kemp, P},
journal = {Journal of Cachexia, Sarcopenia and Muscle},
title = {Pre-surgical miR-675-5p associates with the inflammatory response to surgery and increases inflammatory gene expression in vitro},
year = {2026}
}
TY - JOUR
AB - Background A loss of muscle mass and strength is a marker of poor outcomes following surgery. Changes in muscle gene expression following surgery are likely to contribute to post-surgical muscle loss, but the mechanisms controlling these changes are not known. We have previously shown that miRNAs associate with muscle loss in disease, including surgery, so may contribute to changes in gene expression. We analysed the change in muscle gene expression following surgery and compared it to pre- and post-surgical miRNA expression. We then analysed the effect of one of these miRNAs (miR-675-5p) on gene expression in myoblasts in the presence and absence of an inflammatory stimulus. Methods Differential gene expression analysis was performed on RNAseq data from rectus femoris biopsies (n=18 male patients) pre- and post-surgery. Gene expression was compared with miRNA expression in the same samples to identify patterns of mRNA expression associated with physiology and miRNA expression. The effect of miR-675-5p on gene expression in the presence and absence of TNF-a was determined in LHCN myoblasts by RNAseq. Results Post-surgery muscle had increased expression of gene sets associated with TNF-a signalling (2.7fold) and MYC targets (2.7-fold). Loss of muscle mass and strength associated with MYC target gene sets, but inflammatory gene sets only associated with loss of strength. Comparing miRNA expression with post-surgery gene expression showed that pre-surgery miR675-5p expression was most closely associated with post-surgical gene expression, indicating that the pre-surgery phenotype is important in determining the response to surgery. Pre-surgery miR675-5p correlated with change in gene expression in response to surgery (b=1.30, p<0.001), such that individuals with the highest pre-surgery miR-675-5p had the largest change in gene expression in response to surgery. In LHCN myoblasts miR-675-5p increased CCL2 protein release approximately 1.5-fold (p<0.001). RNAs
AU - Akhundova,N
AU - Paul,R
AU - Fletcher,B
AU - Connolly,M
AU - Griffiths,M
AU - Kemp,P
PY - 2026///
SN - 2190-5991
TI - Pre-surgical miR-675-5p associates with the inflammatory response to surgery and increases inflammatory gene expression in vitro
T2 - Journal of Cachexia, Sarcopenia and Muscle
ER -