See a list of publications below or visit the Photonics academic staff page and click on a particular  member of staff to access their personal web page, which includes a list of their own publications.

Citation

BibTex format

@article{Habte:2026:10.1111/jmi.70148,
author = {Habte, S and Kumar, S and Lightley, J and Garcia, E and Neil, MAA and French, PMW},
doi = {10.1111/jmi.70148},
journal = {J Microsc},
title = {Quantitative evaluation of LED-based optical autofocus module.},
url = {http://dx.doi.org/10.1111/jmi.70148},
year = {2026}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We report an improved version of the open-source optical autofocus (OAF) module (openAF) for light microscopy, replacing the superluminescent diode (SLD) with a multimode-optical fibre-coupled LED as a more accessible AF module light source. We also present a method for independently quantifying AF system performance using 2D autocorrelation analysis of astigmatic fluorescent nanobead images. We applied this method to evaluate both this new LED-based openAF module and our previous SLD-based module implemented on a microscope with a 100 × 1.4 numerical aperture oil-immersion objective lens. Our new approach compensates for AF light source power variation, and we demonstrate that the LED-based system can provide axial stability with a standard deviation below 10 nm for at least 45 min from a cold start as the LED power varies, whereas it reaches thermal equilibrium.
AU  - Habte,S
AU  - Kumar,S
AU  - Lightley,J
AU  - Garcia,E
AU  - Neil,MAA
AU  - French,PMW
DO  - 10.1111/jmi.70148
PY  - 2026///
TI  - Quantitative evaluation of LED-based optical autofocus module.
T2  - J Microsc
UR  - http://dx.doi.org/10.1111/jmi.70148
UR  - https://www.ncbi.nlm.nih.gov/pubmed/42525498
ER  -
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